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KMID : 1132720060040020057
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Genomics & Informatics
2006 Volume.4 No. 2 p.57 ~ p.64
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Single and Dual Ligand Effects on Gene Expression Changes in Mouse Macrophage Cells
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Choi Sang-Dun
Seo Jeong-Sun
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Abstract
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We identified differentially expressed genes in RAW264.7 cells in response to single and double ligand treatments (LPS, $IFN{\gamma}$, 2MA, LPS plus $IFN{\gamma}$, and LPS plus 2MA). The majority of the regulated transcripts responded additively to dual ligand treatment. However, a significant fraction responded in a non-additive fashion. Several cytokines showing non-additive transcriptional responses to dual ligand treatment also showed non-additive protein production/secretion responses in separately performed experiments. Many of the genes with non-additive responses to LPS plus 2MA showed enhanced responses and encoded pro-inflammatory proteins. LPS plus $IFN{\gamma}$ appeared to induce both non-additive enhancement and non-additive attenuation of gene expression. The affected genes were associated with a variety of biological functions. These experiments reveal both dependent and independent regulatory pathways and point out the specific nature of the regulatory interactions
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KEYWORD
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macrophage, gene expression, signal transduction, toll-like receptor (TLR)
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